Development of a novel pulsed electric field generator and a handheld syringe and needle holder with embedded electrodes for electroporation and electroextraction of biological molecules from the tissue

Prof. Alexander Golberg and Prof. Avshalom Shalom

Dep' of Environmental Studies, Faculty of Exact Science and Department of Plastic Surgery, Meir Medical Center and Faculty of Medicine,

Prof. Alexander Golberg
The Porter School of Environment and Earth Sciences Tel Aviv University, Israel. Visiting Scientist
Center for Engineering in Medicine, Massachusetts General Hospital, Boston, USA
Shriners Burns Hospital for Children, Boston, USA

Prof. Avshalom Shalom is an expert on plastic and aesthetic surgery and is among the most senior plastic surgeons in Israel.
Graduated in medical studies from the Tel Aviv University and specialized in plastic surgery at the Asaf ha-Rofe Medical Center in Zrifin. Fellowship of two years at the leading medical centers in the United States, where Prof. Shalom specialized in burns, microsurgery and breast reconstruction.

Additional specialization in dermatoscopy and MOHS surgery from the American ASMS Association.
Today, Professor Shalom heads the Plastic Surgery Unit at the Meir Hospital in Kfar Saba and is a senior lecturer at the Tel Aviv University.

Current solid tissue sampling methods for diagnostics mostly use tissue biopsy, which could lead to localized tissue injury, bleeding, and scarring. Tissue biopsy is followed by pathological examination of the sampled tissue and a decision based on that examination. Recently, we demonstrated novel
technology for harvesting biomolecules in vivo based on extraction using vacuum assisted electroporation – termed e-biopsy. This proposal is based on our previous experience with in vivo usage of pulsed electric field and electroporation technologies.

We propose the following specific aims. Develop a novel pulsed electric field generator for tissue electroporation and electroextraction.
Develop a novel handheld applicator for skin molecular biopsy with electroporation  Validate ebiopsy devices in human non-melanoma skin cancer. In this study, we will use 150 excised human skin samples with known pathology and initially characterized by biomarkers for protein biomarker extraction with the novel equipment. The proposed approach includes temporary tissue permeabilization using non-thermal pulsed electric fields. The permeabilized tissue will release proteins which will be harvested by the same e-biopsy handheld device, pumped out and analyzed for tissue profiling. Profiling will consist of shotgun proteomics. Bioinformatics will be applied to the resulting data to assess and optimize technology performance and to detect differentially expressed proteins and
pathways. In addition, a classifier will be developed that will provide a basis for the potential future use of the proposed technology in diagnostics. This project will be executed as a collaboration between a Prof. Golberg, Prof. Yakhini, Prof. Shalom.

Based on molecular profiles of tumors and other tissues, personalized medicine aims to optimize medical care and preventative measures on an individual patient basis. In cancer therapy and care, a clear potential advantage has been demonstrated to the personalized approach as compared to traditional therapies.
To enable tumor profiling, current methods use tissue biopsy, which involves the physical resection of a small tissue sample. This procedure leads to localized tissue injury, bleeding, inflammation, neural damage, and stress